Journal: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics

Article Title: RNA Interference of Biot2 Induces G₁ Phase Arrest and Apoptosis in Mouse Colorectal Cancer Cell Line

doi: 10.3727/096504014x14146137738583

Figure Lengend Snippet: Figure 2. Biot2 genes downregulated arrest of CT26 cell cycle. The effect of Biot2-shRNA on inducing G1 cell cycle arrest in CT26 cells. After treatment with Biot2-shRNA for 48 h, (A) the cell cycle distributions were determined by flow cytometry. (B) The protein expressions of cycle regulatory proteins and cyclin-dependent kinase cyclin D1, CDK2 and (C) the expression of several CKIs, p16, p21, and p27, were measured by Western blotting.

Article Snippet: Rabbit anti-CDK2 (1:1,000), rabbit anti-p16 (1:1,000), rabbit anti-p27 (1:1,000), and mouse anti-PCNA (1:1,000) were purchased from BOSTER (China).

Techniques: shRNA, Flow Cytometry, Expressing, Western Blot

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Baseline and operative characteristics

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: The median CD73 level before CPB and during rewarming; * P <0.0001. CPB, cardiopulmonary bypass.

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Trend of the median CD73 level over time in subjects with CD73 levels at four time points ( n =18); * P <0.05.

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Preoperative subject characteristics and preoperative CD73 levels

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Baseline and operative subject characteristics and rewarming CD73 levels

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Multivariable modeling of CD73 with VIS at 24 hVIS, vasoactive inotropic score.

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Multivariable modeling of CD73 with ICU length of stay.

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques:

Journal: Pediatric Research

Article Title: Infant cardiopulmonary bypass: CD73 kinetics, association with clinical outcomes, and influence on serum adenosine production capacity

doi: 10.1038/pr.2017.325

Figure Lengend Snippet: Multivariable analysis of CD73, AP, and ex vivo adenosine production capacity.

Article Snippet: CD73 levels from each sample were measured using commercially available enzyme-linked immunosorbent assay kits specific for CD73 (MyBioSource, San Diego, CA).

Techniques: Ex Vivo

Journal: bioRxiv

Article Title: Platelet HMGB1 in Platelet-Rich Plasma Promotes Tendon Wound Healing

doi: 10.1101/2021.04.22.440884

Figure Lengend Snippet: Very low levels of CD73 are found in the wound areas of KO-PRP mouse patellar tendons ( A-F ) compared to GFP tendons, which have much higher levels of CD73 expression( G-L ). However, both KO-PRP treated and GFP-PRP treated wound areas ( C-F , and I-L ) have more CD73 than the wounds treated with saline ( A, B , and G, H ). Semi-quantification ( M ) confirms the results. *P < 0.01 (KO-PRP vs. GFP-PRP). KO-PRP: PRP from PLT HMGB1-KO mice; GFP-PRP: PRP from GFP mice. White bars: 200 μm; Yellow bars: 50 μm (immunostaining).

Article Snippet: Similarly, the fixed tissue sections were reacted individually overnight at 4°C with the following primary antibodies: rabbit anti-CD68 antibody (1:500, Abcam, Cat. #125212, Cambridge, MA), rabbit anti-CD146 antibody (1:500, Abcam, Cat. #75769; Cambridge, MA), rabbit anti-CD73 antibody (1:500, LSBio, Cat. #LS-B14527-50, Seattle, WA), or rabbit anti-collagen III antibody (1:500, ThermoFisher, Cat. #22734-1-AP, Waltham, MA).

Techniques: Expressing, Immunostaining